CHAPTER XXX 

 A CLASS LIST OF PREPARATIONS 



Where a regular course in histology is conducted, it is a good plan to 

 give each student at the outset a complete list of the preparations 

 which he is expected to make. In a three months' course a fairly repre- 

 sentative collection of preparations can be made. The availability of 

 material determines what a list shall be. Besides gaining an introduc- 

 tion to the use of the microscope and its accessories, a class meeting 

 ten hours a week for ten weeks should be able to do as much work as is 

 outhned below. 



In making the mounts, the order indicated in the list should not be 

 followed. Begin with temporary mounts, and then study, in succession, 

 freehand sections, the glycerin method, the Venetian turpentine meth- 

 od, the paraffin method, the celloidin method, and special methods. 

 A large proportion of the time should be devoted to the paraffin 

 method. 



It is neither possible nor desirable that each student should in every 

 case go through all the processes from collecting material to labeling. 

 Some of the material may be in 85 per cent alcohol, some in formalin, 

 some in glycerin, some in Venetian turpentine, and some in paraffin. 

 One student may imbed in paraffin enough of the Anemone for the 

 whole class; another may imbed the Lilium stamens; and by such a 

 division of labor a great variety of preparations may be secured with- 

 out a corresponding demand upon the time of the individual. 



LIST OF PREPARATIONS 

 THALLOPHYTES 



MYXOMYCETES 



1. Trichia varia. — Paraffin sections 5 /j.. Safranin, gentian violet, orange. 



SCHIZOPHYTES 



SCHIZOMYCETES 



2. Bacteria. — Coccus, Bacillus, and Spirillum forms. Stain on cover glass 

 or slide. 



3. Bacillus anthracis. — In liver of mouse. Paraffin sections, 5 m- Stain in 

 gentian violet, Gram's method. 



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