A CLASS LIST OF PREPARATIONS 381 



20. Vaucheria. — Stain in iron-alum haematoxylin and mount in Venetian 

 turpentine. 



21. Chara.— Cut paraffin sections of the apical cell, ooginia, and antheridia. 



PHAEOPHYCEAE 



22. Edocarpxis.— Stain some in iron-haematoxylin and some in phloxine 

 and anilin blue. Mount both together in Venetian turpentine. 



23. Cii<?ena.— Sections of oogonia, antheridia, and sporangia. Cut 10 m thick 

 and stain in iron-haematoxylin with about 7 minutes in safranin. 



24. Fucus vesiculosus. — Antheridial conceptacle with paraphyses and an- 

 theridia; oogonial conceptacle with oogonia. Cut 10 n thick and stain in 

 iron-haematoxylin with about 5 minutes in safranin. For details of 

 antheridia, cut 1 or 2 /z. 



RHODOPHYCEAE 



25. Nemalion. — Stain some in iron-haematoxylin and some in eosin. Each 

 preparation should show trichogyne, carpogonium, and cystocarp. You 

 cannot mount it in Venetian turpentine; use glycerin or glycerin jelly. 



26. Polysiphonia. — Stain in iron-haematoxylin or phloxine and anilin blue. 

 Mount whole in Venetian turpentine. Each mount should show tetra- 

 spores, antheridia, and cystocarps. If material is in paraffin, cut sections 

 about 7 M thick. 



FUNGI 



PHYCOMYCETES 



27. Rhizopus nigricans. — Stain young sporangia in eosin, dilute Delafield's 

 haematoxylin, or in phloxine and anilin blue. Some zygosporic material 

 should be stained strongly in eosin: some should be stained in iron-alum 

 haematoxylin, and in reducing the stain, some should be taken out early 

 to show the coenocytic character of the mycelium, and some should be 

 drawn farther to show the structure of the zygospores and suspensors. 

 Venetian turpentine. 



28. Saprolegnia.— Stain some in phloxine and anilin blue, and some in iron- 

 alum haematoxylin. Each mount should show sporangia and oogonia. 

 Venetian turpentine. 



29. Albugo Candida. Select white blisters which have not yet broken open. 

 Paraffin 8 ^l. Iron-alum haematoxyhn and orange. Oogonia and an- 

 theridia, 2-5 fi, same stain. 



30. Albugo bliti on Amarantus retroflexus. — Cut out small portions of leaves 

 in which the oogonia can be seen in abundance. Paraffin, 2-5 m- 



ASCOMYCETES 



31. Pe^iza.— Paraffin sections of young apothecia, 5 m or less; sections of 

 older apothecia, 10 or 15 ju- Safranin, gentian violet, orange. 



32. Aspergillus, Eurotiimi. — Stain in eosin and mount in Venetian turpentine. 



