382 METHODS IN PLANT HISTOLOGY 



33. Penicillium. — Treat like Aspergillus. 



34. Enjsiphe commune on Polygonum aviculare. — Strip the fungus from the 

 leaf. Paraffin, 5 m or less. Safranin, gentian violet, orange. 



35. Uncinula necator on Ampelopsis quinquefolia. — Stain in phloxine and 

 anilin blue. Mount whole in Venetian turpentine and break the perithecia 

 under the cover. 



36. X?/Zana.— Paraffin sections of younger stages. Delafield's haematoxylin 

 and erythrosin. Be sure that some section in each mount shows the open- 

 ing of a perithecium. 



LICHENS 



37. Physcia stellaris.— Cut in paraffin, 5 m- Stain in cyanin and erythrosin, 



BASIDIOMYCETES 



38. Puccinia graminis.—Aecium stage on barberry leaf. Urediniospore and 

 teliospore stage on oats. Cut 3 m and stain in iron-haematoxylin. 



39. Coprinus wicacews.— Paraffin. Transverse sections of gills showing trama, 

 paraphyses, basidia, and spores. To show the basidium with four spores, 

 the sections should be 15 m thick. For development of the spores, cut 5 fi 

 or less. Safranin, gentian violet, orange. Boletus, Htjdnum, and Polyporus 

 are treated in the same manner. 



BRYOPHYTES 



HEPATICAE 



40. Riccia natans.— Paraffin, 10 or 15 n. Delafield's haematoxylin. Arche- 

 gonia, antheridia, and sporophytes imbedded in the gametophyte. 



41. Marchantia polymorpha.—FsiTaffin, 5 or 10 ju. Archegonia, antheridia, 

 and sporophytes. 



42. Anthoceros /aezii.s.— Paraffin, 5 or 10 m- Longitudinal and transverse sec- 

 tions of sporophyte. Safranin, gentian violet, orange. 



43. Pellia epip/ii/ZZa.— Paraffin, 5 or 10 m- Longitudinal sections of sporophyte 

 attached to gametophyte. Safranin, gentian violet, orange. 



44. Porella platyphylla.—Farsiffin, 10 m- Delafield's haematoxyhn. Arche- 

 gonia, antheridia, sporophyte, and apical cell. 



MUSCI 



45. Sphagnum.— Leai buds. Cut 5 n and stain in safranin and anilin blue. 



46. Sphagnum.— Capsule. Paraffin. Delafield's haematoxylin and erythrosin. 



47. Funaria hygrometrica.—Favaffin. Lorigitudinal and transverse sections 

 of young capsules. Delafield's haematoxylin. 



48. Funaria hijgrometrica or any favorable form. Protonema. Place the well- 

 cleaned material directly into 50 per cent glycerin and allow it to concen- 

 trate. Mount in glycerin or glycerin jelly. 



49. Bryrm.— Paraffin. Antheridia, 10 n; archegonia, 15-20 n; capsule, 10 n. 

 Peristome mounted whole. 



