FORMULAS FOR REAGENTS 387 



Schaffner's chromo-acetic solution. — 



Chromic acid . 3 g. 



Glacial acetic acid 0.7 c.c. 



Water 99 .0 c.c. 



Chromo-acetic solution (for marine algae). — 



Chromic acid 1 • g. 



Glacial acetic acid 0.4 c.c. 



Sea-water 400.0 c.c- 



Material must be washed in sea water. 



Strong chromo-acetic solution. — 



Chromic acid 1 g- 



Glacial acetic acid 3 c.c. 



Water 100 c.c. 



Licent's formula. — 



1 per cent chromic acid 80 c.c. 



Glacial acetic acid 5 cc. 



Formalin 15 c.c. 



Flemming's fluid (weaker solution). — 



1 per cent chromic acid (in water) 25 c.c. 



1 per cent glacial acetic --icid (in water) 10 c.c. 



^ Water 55 c.c. 



B. 1 per cent osmic acid (in water) 10 c.c. 



Keep the mixture A made up, and add B as the reagent is needed 

 for use, since it does not keep well. 



Flemming's fluid (stronger solution). — 



1 per cent chromic acid 45 c.c. 



2 per cent osmic acid 12 c.c. 



Glacial acetic acid . 3 c.c. 



Chicago chromo-acetic-osmic solution. — 



Chromic acid 1 g- 



Glacial acetic acid 2 c.c. 



1 per cent osmic acid 6 to 8 c.c. 



Water 90 c.c. 



Merkel's fluid. — 



1.4 per cent solution of chromic acid 25 c.c. 



1.4 per cent solution of platinic chloride 25 c.c. 



