388 METHODS IN PLANT HISTOLOGY 



Benda's fluid. — 



1 per cent chromic acid 16 c.c. 



2 per cent osmic acid 4 c.c. 



Glacial acetic acid 2 drops 



Hermann's fluid. — 



1 per cent platinic chloride 15 parts 



Glacial acetic acid 1 part 



2 per cent osmic acii 4 or 2 parts 



Nawaschin's fluid. — 



A 



Chromic acid 1 ■ 5 g. 



Glacial acetic acid 10 c.c. 



Distilled water 90 c.c. 



B 



Commercial formalin 40 c.c. 



Distilled water 60 c.c. 



Mix equal parts A and B just before using. Used for mitosis. Try it 

 for embryo sacs and for free nuclear stages in pteridophytes, gymno- 

 sperms, and angiosperms. 



Picric acid. — 



Picric acid 1 g- 



Water or 70 per cent alcohol 100 c.c. 



Bouin's fluid. — 



Commercial formalin 25 c.c. 



Picric acid (saturated solution in water) 75 c.c. 



Glacial acetic acid 5 c.c. 



Corrosive sublimate and acetic acid. — 



Corrosive sublimate 3 g. 



Glacial acetic acid 5 cc. 



70 per cent alcohol (or water) 100 c.c. 



Corrosive sublimate, formalin, acetic acid. — 



Corrosive sublimate 4 g. 



Formalin 5 c.c. 



Glacial acetic acid 5 c.c. 



Water (or 50 per cent alcohol) 100 c.c. 



For material to be mounted in Venetian turpentine, use the aqueous 

 solution ; for imbedding, use the alcoholic. Both this and the preceding 

 solution should be used hot (85° C). 



