FORMULAS FOR REAGENTS 393 



Iodine green. — 



Iodine green 1 g- 



70 per cent alcohol 100 c.c. 



Methyl green. — 



Methyl green 1 g- 



Glacial acetic acid 1 c.c. 



Water 100 c.c. 



If the preparation is to be mounted in balsam, a slight trace of 

 acetic acid and also a trace of methyl green should be added to the 

 absolute alcohol used for dehydrating. 



For staining vascular bundles, the acid may be omitted, even from 

 the formula. 



Light Green. — 



Light green 1 §• 



90 per cent alcohol 100 c.c. 



or 



Light green 1 §• 



Clove oil 100 c.c. 



or 



Light green 1 §• 



Clove oil 75 c.c. 



Absolute alcohol 25 c.c. 



Fuchsin. — 



Fuchsin 1 g- 



95 per cent alcohol 100 c.c. 



Water 100 c.c. 



Gourley's basic fuchsin. — 



Basic fuchsin 50 mg. 



95 per cent alcohol 2 c.c. 



Tap water 100 c.c. 



Dissolve the fuchsin in the alcohol and add the water. Gourleyused 

 two lots of fuchsin, both successful; one from Coleman and Bell, the 

 other from the Will Corporation. 



Acid fuchsin. — 



Acid fuchsin 1 g- 



Water 100 c.c. 



Use this formula when staining woody tissues in methyl green and 

 acid fuchsin. 



