A 

 B 



394 METHODS IN PLANT HISTOLOGY 



Ziehl's carbol fuchsin. — 



Fuchsin 1 g. 



Carbolic-acid crystals 5 g. 



95 per cent alcohol 10 c.c. 



Water 100 c.c. 



Fuchsin and iodine green mixtures. — 



Two solutions are kept separate, since they do not retain their 

 efficiency long after they are mixed. 



J Fuchsin (acid) . 1 g. 



\ Distilled water 50 . c.c. 



j Iodine green . 1 g. 



\ Distilled water 50 . c.c. 



Absolute alcohol 100 . c.c. 



Glacial acetic acid 1.0 c.c. 



Iodine . 1 g. 



Stain in equal parts of A and B. Transfer from the stain directly 

 to solution C, and from C to xylol. 



Another formula. — 



. J Acid fuchsin . 5 g. 



\ Water 100 . c.c. 



j Iodine green . 5 g. 



\ Water 100.0 c.c. 



Mix a pipette full of A with a pipette full of B; stain 2-8 minutes; 

 dehydrate rapidly and mount in balsam. 



Phloxine. — 



Phloxine 1 g. 



85 or 90 per cent alcohol 100 c.c. 



This behaves like the better lots of the stain described under the 

 name of Magdala red, in previous editions of this book, and may be 

 identical with those better lots, 



Safranin. — 



In previous editions it was recommended that in making a 50 per 

 cent alcoholic safranin, two safranins should be mixed, one dissolved 

 in absolute alcohol and the other in water. I am reliably informed 

 that the results averaged better because safranins varied and the 

 method gave two chances to get some good safrunin, instead of only 



