396 METHODS IN PLANT HISTOLOGY 



alcohol; then add 100 c.c. of clove oil. Let the absolute alcohol evap- 

 orate until there is left about 100 c.c. of the solution. 



Gold orange. — 



Gold orange . 1 g. 



Clove oil 100 c.c. 



Dissolve the 0,1 g. of gold orange in 100 c.c. absolute alcohol, add 

 100 c.c. clove oil and let the alcohol evaporate until there is about 100 

 c.c. of the solution. Gold orange dissolves more readily than orange G. 



Bismarck brown. — 



Bismarck brown 2 g. 



70 per cent alcohol 100 c.c. 



Nigrosin. — 



Nigrosin 1 g- 



Water 100 c.c. 



Gram's solution. — 



Iodine 1 g- 



Iodide of potassium 2 g. 



Water 300 c.c. 



MISCELLANEOUS 

 Mayer's albumen fixative. — 



White of egg (active principle) 50 c.c. 



Glycerin (to keep it from drying up) 50 c.c. 



Salicylate of soda or carbolic acid (to keep out bac- 

 teria, etc.) 1 g- 



Shake well and filter through linen. 



Land's gum fixative. — 



Gum arable 1 g- 



Potassium dichromate 1 g- 



Water 98 c.c. 



Dissolve the gum in water and add the dichromate of potash; or 

 dissolve the gum in half the quantity of water and the dichromate of 

 potash in the other half, and mix just before using. Le Page's hquid 

 glue may be used instead of the gum arable. 



