FORMULAS FOR REAGENTS 397 



Haupt's gelatin fixative. — 



Gelatin 1 g. 



Phenol 2g. 



Glycerin 15 c.c. 



Water 100 c.c. 



Dissolve the gelatin in distilled water at 30° C; add 2 g. phenol 

 crystals and the glycerin. Stir well and filter. In smoothing ribbons, 

 flood the sKde with 2 per cent formalin, which makes the gelatin in- 

 soluble. 



Szombathy and also Artschwager have described gelatin fixatives. 



Schultze's maceration fluid. — 



The ingredients are nitric acid and potassium chlorate. They are 

 mixed only as the reagent is applied. See chapter on "Special Meth- 

 ods" (chap. xii). 



Jeffrey's maceration fluid. — 



Nitric acid (10 per cent in water) 50 c.c. 



Chromic acid (10 per cent in water) 50 c.c. 



For directions, see chapter xii. 



Bleaching fluid. — 



Potassium chlorate (dry) . 1 g. 



Hydrochloric acid 0.5 c.c. 



Alcohol (30 or 50 per cent) 100 c.c. 



Put the potassium chlorate on the bottom of a dry Stender dish, 

 drop the hydrochloric acid on with a pipette, and after 20 or 30 

 seconds pour on the alcohol. 



This can always be made fresh and is as good or better than hydro- 

 gen peroxide for bleaching osmic acid material. 



Fehling's solution. — 



. J Cupric sulphate 3 g. 



^ \ Water 100 c.c. 



f Sodium potassium tartrate (Rochelle salts) ... 16 g. 



B 



c.c. 



(Water 100 



J Caustic soda 12 g- 



^ { Water 100 c.c. 



Keep it in three bottles labeled A, B, and C. When needed for use, 

 add 10 c.c. of water to 5 c.c. from each of the three bottles. 



