29 



B. Rumberg, P. Schmidt-Mende , J. V/eikard and H.T. Witt 



Fip;.14. Absorption changes at 7o5 mu , 25^ mu and 515 mu in spi- 

 nach chloroplast fragments as function of different degrees of 

 extraction with petroleum ether. 2o C, pH = 7»2. 



Fig. 15. Rate of Op-production by illuminated spinach chloro- 

 plast" fragments and magnitude of the absorption changes at 515 

 mu in the same preparation of chloroplast fragments. Normal con- 

 dition, after extractions with petroleum ether different degrees, 

 and following recondensation of plastoquinone . 15 C; pH = 7>2; 

 ferricyanide was added as Hill oxidant. 



Fip;.16. -faction spectra of reaction cycles I and II. Top: Measur- 

 ed by the magnitude of the absorption changes at ^35 mu in aged 

 spinach chloroplast fragments in the presence of 1o~^ M/1 PMS 

 plus I0-2 M/1 ascorbate (2o"C, pH = 7,2) and at 515 mu in normal 

 spinach chloroplast fragments in the presence of 1,5.1o ^ M/1 

 DFIP plus I0-2 M/1 ferricyanide (2o C, pH = 7,2). Bottom: Measure 

 ed by the rate of Op-production of illuminated spinach chloro- 

 plast fragments in the presence of 2.1o"5 M/1 DFIP plus 6.10"-* 

 M/1 ferricyanide (2o 0, pH = 7,2) and measured by the rate of 

 0^-consumption of illuminated aged spinach chloroplast fragments 

 ife the presence of lo"^ M/1 DFIP plus 5-1o ^ M/1 ascorbate plus 

 5.1o"5 \,/\ benzylviologene (2o G, pH = 8). (Measured by U.Siggel 

 in our laboratory) . 



Fig. 17. Top: Time course of the absorption changes_at ^3o mu in 

 spinach chloroplast fragments in the presence of I0 ^ M/1 benzyl- 

 viologene illuminated with 72o mu-light (1/2 sec), followed by 

 658 mu-light (10""^ sec) (2o°C, pH = 7,2). Bottom: Time course of 

 the absorption changes at 25-^ mu in chlorella cells illuminated 

 with 658 mu-light (1/5 sec), followed by 72o mu-light (1 sec) 

 (2o^G, pH = 7,2). 



Fig. 18. Top: Absorption changes at 515 mu and 7o5 mu in whole 

 spinach chloroplasts in the presence of 1o~^ M/1 ferricyanide as 

 function of aging. (2o C, pH = 7,2). Bottom: Absorption changes 

 at 515 mu and 7o5 mu in whole spinach chloroplasts in the pre- 

 sence of 1o~^ M/1 ferricyanide as function of pH (2o C). 



Fig. 19. Half-life of the absorption changes at 515 mu and 7o5 mu 

 (strong measuring light) in chlorella cells in dependency of 

 temperature (pH = 8). The chlorella culture differs from that 

 used in the measurements of fig. 5* (In chloroplasts identical 

 kinetics at 515 mu and 7o5 mu could as yet not be established). 



Fig.2o. Absorption changes at 515 mu and 7o5 mu in whole spi- 

 nach chloroplasts in the presence of I0 o M/1 indigocarmine as 

 function of concentration of added ClllU. (2o G, pH = 7,2). 



Fig. 21 . See text. 



