237 



Helen M. Habermann 



10 



o 



2 • 



10"* I0-' 



10" 



10" 



I 



/' Chlorogenic ocid 

 extracted 



500 

 O 



■ 400 

 300' 

 200 

 100 



o 



Concentration of cyanide (Molar) 



0(Ui51 



20 40 60 80 100 120 



Seconds in boiling water before extraction 



-I 1 1 r 1 1 T" 



conlrols 



10 20 30 40 50 60 70 

 ;jMoles chlorogenic ocid 

 added per gm leaf 



Fig. L (upper Left). Effects 

 of cyanide in the extracting 

 buffer on allagochrome values 

 of node 3 sunflower leaves. 

 Vertical bars through points 

 indicate standard deviations 

 of means of six determinations. 



Fig. 2 (upper right). Effects of immersion in boiling water prior 

 to extraction on allagochrome values of node 8 sunflower leaves. 

 Chlorogenic acid removed was estimated from optical density of 

 aqueous extracts. 



Fig. 3 (bottom). Effects of heating on the secondary synthesis 

 of allagochrome from chlorogenic acid added to the extracting 

 buffer. Leaves were immersed in boiling water for 10, 20, or 30 

 sec. before extraction. 



