671 



Martin Gibbs 



Table 5- DISTRIBUTION OF C IN GLUC0SE-6-P, 

 FRUCTOSE- 1,6-diP, AND GLYCERATE-3-P ISOLATED FROM RUPTURED PEA 

 CHLOROPLAST SYSTEM AFTER 40 MINUTES OF INCUBATION 



.1^ 



Ik 



rate when compared to the intact cell. All attempts to increase 

 the basic rate of COp reduction by the intact higher plant chloro- 

 plast in this laboratory and apparently in others have resulted 

 in uniform failure. It would appear that the present methods of 

 preparing chloroplasts, 0.35 M NaCl, O.k M sucrose, or density 

 gradient, are not suitable for elucidation of the path of carbon. 

 Even though isotope spreads rapidly throughout the carbon atoms 

 of the hexose in the intact chloroplast, the low overall rate is 

 still an important problem that cannot be ignored. The data 

 obtained with these preparations substantiate the observation of 

 Bassham and Kirk'^ that differences may occur between the intact 

 and fragmented systems. Integrity of a multifunctional enzyme 

 system may be a key factor in elucidation of the cycle. 



Inhibitor data 



The site of iodoacetamide inhibition has been reported to 

 phosphoribulokinase ' while /the site of arsenite inhibition 

 cannot be assigned at present ' . 



(21 22) 

 D-threose-2,i*-diP has been reported^ ' to specifically 



