696 



Shigetoh Miyachi 



with the increase with poly-P^^ "A" could not be detected, because the 

 increment in P-^^ in poly-P-^^ "A" itself is too small as compared with 

 the amount of poly-P"C". By the short term experiment it has been found 

 that incorporation of orthophosphate-P^ into poly-P"B" and lipid-P takes 

 place independent of light at least for a limited period, whereas incorpor- 

 ation of ortho-P into poly-P"C" occurs only in light' ^ The schematic 

 representation on the formation and transformation of respective poly- 

 phosphates and their probable relationship to the syntheses of nucleic 

 acids, P-protein and P-lipid as elucidated thus far is shown in Fig, 5. 



The responses of polyphospiiates to light seems to indicate different 

 locations of each polyphosphate within the Chi ore 11a cell. From the facts 

 that the formation of poly-P"C" and the mobilization of P in poly-P"C" for 

 the syntheses of other P-compounds are induced by light, we are tempted 

 to presume that it exists in or very near to the chloroplast. Our pre- 

 liminary study with Chlorella' ^ showed that the volutin granule consists 

 of poly-P"A" and RNA but it could not be decided whether poly-P"C" 

 exists in chloroplasts or not, although labile P-compounds were detected 

 in the poly -P"C" -fraction of chloroplasts. It has been also found that 

 acid -insoluble inorganic polyphosphate exists in spinach leaves' ^ This 

 suggests that the presence and function of polyphosphates are not limited 

 to lower plants but they are playing an active role in the photo -induced 

 intracellular phosphate transfer in green cells in general. 



ACKNOWLEDGMENT 



The research reviewed in this paper has been the result of collobora- 

 tion with Prof. E. Hase, Dr. R. Kanai, Miss S. Mihara, Mr. S. Aoki 

 and Mrs. S. Miyachi. 



[rna-p] 



["Lipid-pl 



-^ Dor k Reaction 



=> Light Reaction 



Fig. 5. Incorporation and mobilization of P in Chlorella cells 



