715 



William F. Prickett, F. Dudley Bryant, and Paul Latimer 



absorption band structure. There is indeed precedence for this type of ap- 

 proach to absorbance spectroscopy'^^'. While the theoretical scattering 

 curves I and II, based on the assumption of one and two components only of the 

 chlorophyll a band, give very sinnilar theoretical scattering curves, we note 

 that scattering curve III, based on the assumption that there is a significant 

 band at 697 mp, clearly reflects the presence of this absorption band. No ex- 

 perimental scattering spectrum to date has shown clear evidence of such a 

 band. 



The present data is not sufficiently precise to exclude the existence of a 

 small chlorophyll a band m vivo near 700 mji. However, this neglected ap- 

 proach to absorbance spectrophotometry of living nnaterials warrants further 

 exploration. More precise measurennents of scattered light are needed. 



This work was supported by research grant GM- 10922 from the U. S. 

 Public Health Service. We wish to thank Mr. J. E. Johnson, Miss Vivian 

 Griffin and Mr. L. Patrick for technical assistance, and Dr. N. K. Sanyal 

 for helpful suggestions. 



W. F. Prickett, Senior Thesis, Vanderbilt University, Nashville, Ten- 

 nessee (1963). 



P. Latimer, in Studies of Microalgae and Photo synthetic Bacteria , 

 (Japanese Society of Plant Physiologists, eds. , ), p. 213, University of 

 Tokyo Press, Tokyo, 1963. 

 van de Hulst, H. C. , Light Scattering by Small Particles , p. 172, John | 



Wiley and Sons, New York, 1957. | 



MacRae, R. A. , McClure, J. A. , and Latimer, P., J. Opt. Soc. Am. 

 5j_, 1366 (1961). 

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(1959). 



Latimer, P., Plant Physiol. 34, 193(1959). 



Charney, E. , and Brackett, F. S. , Arch. Biochem. Biophys. 92, 1 (1961). 



Lothian, G. F. , and Lewis. P. C. , Nature, 178, 134^^(1956). 



Latimer, P., J. Opt. Soc. Am. 5J_, 116(1961). 



Robinson, T. S. , and Price, W. C. , Proc. Phys. Soc. (London) B66 , 969 



(1953). 



