50 



P. Larsen and T. Aasheim 



From these results, the identity of the neutral auxin in etiolated 

 pea epicotyls with lAAld is indicated by R^ (0.055) , color reaction 

 with DMCA (clear blue) , and reaction toward soil treatment (several- 

 fold increase in auxin activity). Obviously, this auxin cannot possibly 

 be identical with IAN. 



Brassica. Samples representing 0.5 to 0.6 g. fresh weight were ap- 

 plied as described above for Pisum, and the chromatograms were 

 treated and eluted in the same manner. Results are shown in Figure 2. 



0.05 



0.10 



0.15 



Rf 



Fig. 2. .'Vuxin activity of eluates of chromatograms of extracts of Pisum and 

 Brassica. Solid lines represent activity olitaincd directly and broken lines after 

 treatment with soil. Areas corresponding to the indole-3-acetaldehvde in Pisum 

 show a considerable increase in activity after treatment in soil. For further expla- 

 nation see text. 



Colored spots dc\el()ped in the follow iiig places after spra)ing Avilh 

 DMCA: starting line (reddish purple), Rf 0.10 (purple), Rf 0.135 (pur- 

 ple) . In addition to these spots a faint, reddish-pinple spot was de- 

 tected at Rf 0.4 on a chromatogram which was run for Si/ hrs. instead 

 of 18 hrs., and in which the front had moved to 32.7 cm. 



Bioassays were made on zones III and IV in the IS lir. chromato- 

 gram (Figure 2) and very high activity was fouiul in botli. (Note the 

 different scales for Brassica and Pisum.) Supramaxinuini cmvatines 

 were obtained from /one IV at the concentrations tested, but no at- 



