Neio Auxins From 'Maryland Mammoth' Tobacco 



59 



way with ethanol, followed by removal of the alcohol, extraction of 

 the residual solution with ether, and chromatography of the two 

 layers separately on paper using isopropanol-ammonia-water (80:5: 

 15) as solvent. Figure 1 reveals the presence of ether-soluble growth 

 substances having Rf values identical with those of lAA and IAN. 

 However, the area of greatest growth-promoting activity (R^ 0.5 to 0.6) 

 failed to give the typical indole color reactions, while the areas above 

 Rf 0.9 gave only a very faint color, as described previously (11). 



o 



o 

 o 



o 



UJ 



o 



QL 

 UJ 

 QL 



x" 



t- 



o 

 q: 



CD 



320 



240 



160 



80 







lAA 



N. TABACUM 



IAN 



0.2 



0.4 



0.6 



0.8 



1.0 



Rf 



Fig. 1. Ether-soluble growth substances from N. tabacum L., 'Maryland Mammoth/ 

 as detected by the Avena first internode assay. The shaded areas indicate responses 

 significant at the 1 per cent level. 



