Chromatograpliic Investigations of Indole Compounds 



79 



ICA 



I PA 



IVA ICAPA IHA " W 



I 



w 



w 



SOLVENT FROHT 



ICA lAA IP* IBA IVA IC^PA IHA W 



B 



SOUV£UT FRONT 



Fig. 1. Chromatograms of extracts of (A) wheat-treated and (B) pea-treated solu- 

 tions of w-(indole-3-)alkanecarboxylic acids sprayed with EhrHch's reagent. See 

 Table 1 for chemical names corresponding to the abbre\iations. 



fore, that the compounds of low Rf present in our extracts of pea- 

 treated acetic and propionic acids represent the products of an in vivo 

 condensation reaction between aspartic acid and each of these indole 

 acids. In support of this, the compound obtained from the metabo- 

 lized acetic acid was shown on further chromatographic analysis to 

 behave as authentic indole-3-acetylaspartic acid. Furthermore, bio- 

 assay of a chromatogram containing the extract from the metabolized 



