CInomatographic Investigations of Indole Cotnpoiinds 



87 



ACN 



PCM 



VCN 



CCM 



w 





cm 



Acw 



bCvj VCM 



Hew 



B 



Sou\ £NT 



FUCKIT 



Fig. 7. Chromatograms of extracts of (A) wheat-treated and (B) pea-treated solu- 

 tions of a;-(indole-3-)alkanenitriles sprayed with Ehrlich's reagent. See Table 1 for 

 chemical names corresponding to the abbreviations. 



higher acids, was clearly revealed. It is of further interest to note 

 that the chromatographic and bioassay results with the wheat-treated 

 aceto-, butyro- and capro-nitriles revealed traces of a compound at 

 Rf 0.04 which from its Rf, color reactions and biological activity 

 would appear to be indole-3-acetylaspartic acid. 



As previously observed (7, 8) , indole-3-acetonitrile is converted in 

 wheat tissue not only to the corresponding acetic acid but also to in- 

 dole-3-carboxylic acid. The latter reaction, which involves the conver- 



