Changes in Buds and Seeds in Response to Chilliyig 107 



On the other hand, in the seed of cocklebur, we were able to get a 

 very marked correlation. For example, oxygen will break the dor- 

 mancy of dormant cocklebur, but the inhibitor disappears 30 hrs. 

 after you have put them into oxygen, before there are any visible 

 signs of germination. There, the inhibitor disappears before germi- 

 nation. 



Dr. Burstrom: Do your results imply that your promoters and in- 

 hibitors are active in the soluble state, since you have only studied 

 the soluble fractions, contrary to auxins which probably are active 

 in some bound form or other? 



Dr. Wareing: Well, it's very difficult to answer Professor Bur- 

 strom's question in the present state of our knowledge. In fact, I 

 would almost be inclined to throw it back at him and ask him how 

 he would demonstrate this. 



Dr. Wain: Just a very small point on technique here. In the 

 early part of your report, the dry seeds were extracted with water and 

 no inhibitor was found. Then when you soaked the seed and ex- 

 tracted with water, you found an inhibitor. It does seem to me that 

 this might be explicable in terms of ease of extraction. 



Dr. Wareing: I don't think so. Professor Wain. If you extract the 

 dry seed at 0° C, you get no inhibitor. If, however, you keep the 

 seeds soaking for 24 hrs. at laboratory temperature, you get an inhib- 

 itor. On the other hand, if you keep them soaking for 24 hrs. at 0° C. 

 no inhibitor appears. We were very conscious of the possibility that 

 these effects were simply the result of extraction technique, but fur- 

 ther consideration seems to leave no doubt that the inhibitor is met- 

 abolically produced shortly after the seed is soaked at laboratory tem- 

 peratures. 



