190 



Bitancourt, Nogueira, and Scliwarz 



Fig. 6. Double chromatogratn of ultraviolet decomposition products of an alde- 

 hyd;c indole derivative (lA, see footnote 1). Ascending chromatography. Acetone and 

 water 8:2 in both directions in an atmosphere saturated with vapors of acetic acid. 

 A, distribution of spots after the first run. B, after the second run. The diagonal 

 of the chromatogram is indicated by an interrupted line. The center of the primary 

 spots of the presumed polymers of lA is indicated by large dots, numbered I to VI; 

 that of tlie secondary spots (of polymers formed by polymerization or depoly- 

 merization during chromatography) by small dots. lA occupies the large spot 2 and 

 its extension 2'. The outline of spot 2 was traced tiianks to its quenching of the blue 

 fluorescence of tlie fdlcr j)aper under short-wave ultraviolet liglu. After spraying 

 the chromatogram with Salkowski's reagent, the whole area of spot 2 and its exten- 

 sion 2', inclusive of tlic part with a yellow fluorescence (spot 7'), was colored 

 orange-yellow. No reaction was produced at the sites of the primary spots and 

 some of the secondary spots of the presumable polymers showing that they were 

 completely transformed by polymerization or dcpolymerization during the second 

 chromatography. Conventional hatching indicating approximate fluorescence 

 colors of the decomposition products (spots 1, 3, 4, 6 and 7) as in Figure 7. 



a lion. Neutral substances which are not affected by pH form a 

 straight zone (Figure 2). Wavy patterns were also exhibited by com- 

 pounds like lAAL and lA with no strong acidic or basic groups and 

 lAA with water as the solvent, showed at least three different Rf val- 

 ues. These two results are interpreted as possibly indicating forms 

 of dissociation of the carbonyl and imino groups. 



Ether or ethyl acetate fractionation of the decomposed solutions 



