50 to 200 5 mm. long sub-apical pea stem sections 



Crushed in pre-chillcd mortar and honiosrcnizcd 

 with 8 to 16 ml. of 0.25M sucrose + O.OOlAf EDTA. 

 Clentrifuged 30 min. at 600 x gravity. 



^ 



CELL \\ ALLS 



and 

 TISSUE FRAGMENTS 



Si 



CHLOROPLASTS P.. 



Centrifuged 15 min. at 1,500 X gravity 



MITOCHONDRIA P3 S3 



Centrifuged 15 min. at 12,000 X gravity 



Centrifuged 60 min. at 24,000 X gravity 



MICROSOMES 



P4 



1 

 S4 



FINAL SUPERNATANT 



Aliquots 

 for: 



PROTEIN PRECIPITATION DIALYSIS 



Fig. 1. Fractionation scheme for green pea stem sections. 



