Gibberellin-Auxin Interaction and Metabolic Basis 



621 



by centrifugation, and 1.2 ml. of ihe total of ca. 2.5 ml. then used in 

 the electrophoretic separation. 



The electrophoretic peroxidase patterns are shown in Figures 1, 

 2, and 3. Figure 1 shows the developmental pattern of peroxidases in 

 the normal leaf sheath as it grows from a length of 12 mm. to a length 

 of 70 mm. in the course of 6 days. Clearly, there is initially a single 

 major peak, which gradually becomes more complex until there are 

 ultimately at least five major components. Pyrogallol and guaiacol 

 give the same general spectrum, differing only in the relative height 

 of the various peaks (Figure 2). 



Figure 3 shows that at the latest stage of development studied. 



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< 



iij 



CO 



< 



Q 

 X 



o 

 a: 



DWARF 



NORMAL 



10 



20 



DISTANCE CM. 



Fig. 3. Electrophoretic pattern of peroxidases from leaf sheath of normal and 

 dwarf corn. Guaiacol substrate. 



