Studies With C'-labeled Ethylene 



763 



designated X and Y, in four of the solvents giving good separation 

 are simimarized in Table 2. When the aqueous extract was radio- 

 chromatographed in two dimensions following Bassham and Calvin's 

 technique (4, pp. 17-24), at least 9 radioactive spots, with a major 

 metabolite in the general area of the phosphorylated compounds, 

 were demonstrated (Figure 6, 100). Two minor spots in the region 

 where organic acids migrate and three others where amino acids 

 usually appear were found (4). 



Fig. 6. Radiochromatograms of the radioactive fractions from Coleus in six solvent 

 systems (Nos. 94, 95, 96, 97, 98, 99). In each case No. 3 is the water-soUible extract 

 and No. 4 is the fraction soluble in petroleum ether (pigments). No. 100 — two 

 dimensional radiochromatogram of aqueous extract from Coleus, according to 

 Bassham and Calvin's technique, showing multiple radioactive metabolites. 



