2.71 gms. Lyo])hilized Cotton Leaves 



Containing 10.8 X 10*' c. p.m. 



'(100%) 



Ether Extraction 



Residue (59%) Filtrate (1 1 %) (I, II, III, IV, V) 



80%, Ethanol extract 



Nao CO., 



Residue (53.1%) Filtrate (5.6%) 



\A^ater extraction 



(6.9%) 



Amino acids 



(2.4%) 

 (VI) 



Organic acids 



Residue (31.7%) (2.9%) 



; Filtrate (9.6%) Anion 



Takadiastase Soluble proteins exchange 



hydrolysis (IX and X) Dowex 2 



Residue (12.9%) 

 HCI hydrolysis 



Filtrate (5.1%) 

 HCI Autoclave 



Soluble 

 sugars 



(2.5%) 



Residue Hemicellulose 



I 

 H2SO4 hydrolysis 



Evaporate 

 to small 

 volume, 

 dissolve 

 in pet. 

 ether, 

 partition 

 with 90% 

 EtOH 



Organic 



acids Sugars, etc. 



(VII) (2.4%) 



(VIII) 



Xanthophylls Other 

 and lipids pigments 



(3.6%) 



I 

 Cellulose (0.3%) 



Lignin (0.1%) 



Fig. 7. Fractional extraction survey scheme of radioactivity in cotton leaves after 

 15 hrs. exposure to ethylene-C". 



* The materials which adsorb to the anion exchange column cannot be eluted 

 with IN NH4OH. 2N HCI causes a loss of the radioactive carbon. 



