140 



COySR-GLASS I^ITH 

 SECTIONED POLLEN 

 FRAGMENTS EMBED- 

 DED IN GLYCERINE- 

 JELLY. 



LAVATERA 

 PALLIDA 



SECT/0/^ 

 THICHNESSO^Sm, 



ruCHSIN 



!<- /(ff/W/v,. -4 



PARAFINIZED 

 AREA UNDER 

 A COI/ER-GLASS 



Fig. 265. Microscope slide with sectioned pollen walls. 



check under the microscope can be made in order to get an idea of the 

 quality of the sections. 



Further treatment depends on the number of sections transferred to the 

 slide. If there are only a few it is better to allow the liquid on the slide to 

 dry in the air. If the slide contains a great number of sections it is better 

 to wash it with one or two drops of absolute alcohol. Although this proce- 

 dure will probably wash away a number of the sections it is, nevertheless, 

 worth while because the alcohol will separate the sections from each other 

 and, at the same time, remove any dust etc. on the slide. When dry, the 

 slide is rinsed once more with absolute alcohol. After the alcohol has 

 evaporated the plastic is dissolved in acetone. 



The dissolving of the plastic, staining and mounting 



The acetone usually dissolves the plastic in a few hours (generally, the 

 slide is left over-night in the acetone). After inspection under the micro- 

 scope at low magnification (about x 60) good section-bearing areas are 

 marked with a diamond. The slide is then again carefully rinsed with 

 absolute alcohol and with slowly running water. If the sections are not to 

 be stained, the slide is ready for mounting. 



Staining with fuchsin, safranine, or methylene blue is useful, and in 

 fact often necessary, particularly if the thickness of the sections is only 



