5i6 RESEARCHES ON FUNGI 



Through the kindness of Dr. N. J. Gidclings, on April 25, 1924, 

 I received from West Virginia a jjacket of freshly gathered Juniper 

 twigs bearing numerous Cedar apples. Some of these galls were 

 soaked in water for 1 hour, some for 2 hours, and some for 3 hours, 

 whilst others were sprayed with tap- water splashings for 2 hours. 

 Whilst in contact with the water, the teleutosporic horns became 

 greatly swollen (Fig. 209). After the moistening periods were over. 



Fig. 209. — Gymnosporangium Juniperi-virginianae on Juni- 

 perus virginiana. Left, a Cedar apple, after absorption 

 of water, with each teleutospore-sorus now extended 

 into a long gelatinous horn bearing the teleutospores. 

 West Virginia material received in Winnipeg, April 25, 

 and then revived by soaking. Right, a spore-deposit 

 of red basidiospores (sporidia) which accumulated in 

 one night under the horns of a Cedar apple like that 

 shown on the left. Natural size. 



the galls were set in glass chambers. By the next morning all the 

 galls had produced orange spore-deposits (Fig. 209), thus proving 

 that the teleutospores had germinated and had given rise to normal 

 basidia and basidiospores. 



About six hours after some Cedar galls had been sprayed, I cut 

 off a gelatinous horn from one of the galls, placed it in a glass-ring 

 cell, covered the cell with a cover-glass, and then, using the low 

 power of the microscope, observed what was happening. At once 

 it could be seen that a considerable number of teleutospores had 

 developed short basidia which were turned back toward the surface 

 of the horn, and that the sterigmata and basidiospores were on the 

 outer convex side of each basidium and were therefore directed 



