194 



RESEARCHES ON FUNGI 



Methods. — A spore-deposit of a fruit-body of Coprinus lagopus 

 which came up spontaneously in the laboratory on unsterilised 





\ 



#%•% 



Fig. 110. — Coprinus lagopus, a heterothallic Hymenomycete. Photomicrograph of 

 part of a spore-deposit from a small fruit-body which came up spontaneously 

 on imsterilised horse dimg at Winnipeg ; spores immersed in water. The 

 spores, morphologically, are all alike ; but, sexually, as may be determined by 

 mating the mycelia which they produce, they are divisible into four distinct 

 groups to which have been assigned the symbols (AB), (ab), (Ab), and (aB). 

 Magnification, 650. 



horse dung was collected on a clean sterihsed glass shde (c/. Fig. 110). 



A number of spores were then isolated and sown by the dry-needle 



method ^ at intervals in a Petri dish containing cleared sterihsed 



1 W. r. Hanna, " The Dry-Needle Method of making Monosporous Cultures of 

 Hymenomycetes," Annals of Botany, vol. xxxviii, 1924, pp. 791-795. 



