196 RESEARCHES ON FUNGI 



pan with 1000 cc. water. The mixture was boiled for fifteen minutes 



Fig. 112. — Coprinus lagopus. Hyphae at the periphery of a large haploid 

 myceUum like those shown in Fig. Ill (actually No. 2 of Table I), drawn 

 with the camera -lucida. The mycelium was grown on cleared dung-agar, 

 and only the hyphae growing at the surface of the medium have been 

 drawn ; lateral hyphae which dipped down into the substratum are 

 shown cut off by a line ; the cross-walls (for optical reasons) could not 

 be seen and those shown have been added semi-diagrammatically. The 

 haploid nature of the mycelium is indicated by the simple septa, the 

 absence of clamp-connexions, and the wide-angled mode of branching 

 of the leading radial hyphae. The hyphae are as yet all too young to 

 have developed oidiophores and oidia, but these would begin to appear 

 on the older parts of the mycelium here shown about 24 hours later. It 

 will be seen that the lateral branches are more or less tangentially dis- 

 posed and are effecting anastomoses between one another and the radial 

 hyphae, thus converting the mycelium into a three-dimensional network : 

 already seven hyphal fusions, / /, have been made and many more would 

 be made in a short time. Thus, paths which may be used by nuclei of 

 opposite sex when travelling througli the mycelium and diploidising it are 

 being rapidly constructed. At a a a three lateral hyphae, in the course 

 of their growth at the surface of the dung-agar, have each met with a 

 radial hypha, have thereby been deflected from their original course for 

 a time, and then have suddenly resumed their old direction of growth. 

 This curious phenomenon is not infrequently to be observed in cultures 

 of the kind here described. Drawn by A. H. R. BuUer and Ruth 

 Macrae. Magnification, 88. 



and then filtered through cheese-cloth. Water equivalent to that 

 lost through boiling and filtering was then added (so that the volume 



