igS 



RESEARCHES ON FUNGI 



for cultivating monosporous mycelia and for pairing two mycelia 

 equal in size ; and (2) 15 cm. in diameter, for pairing one very large 

 piece of mycelium (4-6 cm. in diameter) with another very small 

 piece of mycelium. 



Criteria of Sex. — In a haploid mycehum of Coprinus lagopus : 



Fig. 114.— Coprinus lagopus. 

 A hypha of a haploid my- 

 celivim, derived from a 

 single basidiospore and 

 growing in dung-agar, 

 which has given rise to an 

 oidial fructification. This 

 fructification is aerial, and 

 it consists of an oidiophore 

 crowned with a drop of 

 liquid containing numer- 

 ous oidia. The oidia, which 

 float freely in the drop, 

 were formed by the break- 

 ing into segments of a 

 number of oidial branches 

 which grew out from the 

 apex of the oidiophore. 

 Drawn, under the direction 

 of the author, by H. J. 

 Brodie. Magnification, 933. 



Fig. 1\5.— Coprinus lagoptis. 

 An oidiophore, like that 

 shown in Fig. 114, which 

 has been submerged in the 

 film of water covering the 

 surface of the dung-agar in 

 which the mycelium grew. 

 The oidia, which were in 

 the drop that previously 

 crowned the apex of the 

 oidiophore, are now scat- 

 tered in the film of water 

 and can be readily seen ; a, 

 the rounded apex of the 

 oidiophore ; b, the oidia. 

 Drawn, under the direction 

 of the author, by H. J. 

 Brodie. Magnification, 933. 



( 1 ) the nuclei occur singly in the hyphae ; (2) the septa are simple and 

 devoid of clamp-connexions ; (3) the lateral branches of a leading 

 radial hypha come away at a relatively wide angle, 40°-90°, varying 

 even past 90° up to 110°; (4) the mycelium develops numerous 

 aerial oidiophores each crowned by a drop of liquid containing 

 numerous oidia ; (5) the aerial mycelium, as viewed with the naked 

 eye, is less dense, less protuberant, and less conspicuous than that 



