208 



RESEARCHES ON FUNGI 



A piece of the haploid mycelium No. 5 (ab) was allowed to grow 

 in a large 15-cm. Petri dish for nine days. At the end of this time, 

 it had attained a diameter of 5 • 5 cm., whereupon it was inoculated 



(ABWaW 



Fig 



121. — Coprimis lagopus. The diploidisation of a large haploid 

 mycelitun by moans of a small diploid mycelium deposited at 

 the centre of the former. A haploid mycelium {ab) was allowed 

 to grow on dung-agar in a large Petri dish for nine days, by 

 which time its periphery had extended to the inner darker 

 circle. It was then inoculated at its centre with a small piece 

 of dung-agar (cross-hatched) containing the diploid mycelium 

 (AB) + (ab). At the end of five days after inoculation, the 

 central (ab) mycelium had extended its periphery as far as the 

 cuter circle, and all its leading radial hyphae had become 

 completely diploidised (as shown by clamp-connexions). Thus 

 the central diploid inoculum (AB)-\-{ab) had diploidised the 

 large haploid (ab) mycelium and had converted it into a diploid 

 mycelium (AB)-\~(ab). Natural size. 



at its centre (Fig. 121) with a small piece of agar (10 square mm.) 

 containing the diploid mycelium No. 10 x No. 5, {AB) + {ab). 



At the end of five days after the small diploid mycehum 

 {AB)Ar{ab) had been placed on the central part of the large 

 haploid mycelium {ab), clamp-connexions were observed all around 



