220 RESEARCHES ON FUNGI 



daily to find out how far on each side of the inoculum along the 

 periphery of the large central haploid mycelium clamp-connexions 



Fig. 127. — Coprinus lagopus. The diploidisation of a large haploid mycelium (Ab) 

 by a small diploid mycelium (Ab)-\-{aB). Tlie large haploid mycelium was 

 allowed to grow on cleared dung-agar until it was 6 • 2 cm. in diameter. It was 

 then inoculated at its periphery with a tiny hyphal mass of {Ab)-]-{aB) 

 mycelium which can be observed on the right-hand side of tlie pliotograph about 

 1 • 5 cm. from the periphery. At the end of 90 hours after inoculation, the 

 large haploid myceliimi had become completely diploidised by the diploid 

 mycelium, as shown by the appearance of clamp-comiexions on all its peri- 

 pheral leading radial hyphae. The pliotograph was taken five days after 

 inoculation and a day and a quarter after the large haploid myceliiun had 

 become completely diploidised. The darkness of tlie central part of the 

 mycelium is due to the aerial hypliae liaving become submerged in liquid 

 excreted by oidiophores when the mycelium was in tlie haploid state. The 

 hyphae which compose the outer fluffy white ring are diploid and bear no 

 oidiophores. Natural size. 



had been formed, and these points were marked on the under side 

 of the plates. 



