222 



RESEARCHES ON FUNGI 



the other side {vide the broken lines in Fig. 128). The limits of the 

 progress of clamp-connexion production are shown in Fig. 128 not 



1 1 Ill 



1 cym. Z 



4Z novju) 



6Z ruruAX) 



Wut ihcun 



Fig. 128. — Coprinus lagopus. The diploidisation of a large haploid myceliiim {AB) 

 by another haploid mycehum {ab) of opposite sex. Tlie circles 2-12, originally 

 drawn in blue pencilon the under side of the Petri dish, show the boundary 

 of the mycelium from the end of the second to the end of the twelfth day. 

 The {AB) mycelium was inoculated with a tiny hyphal mass of an {ah) mycelium 

 after nine days of growth (periphery shown by the heavier inner circle. No. 9) 

 at the zero hour. The {ab) mycelium diploidised the {AB) mycelium in the 

 course of three days. The crosses indicate where clamp -connexions were 

 observed after 42, 50, 52, and less than 64 hours, respectively. The {ab) nuclei 

 must have travelled (see upper broken line) more than 6 cm. or 60 mm. through 

 the {AB) hyphae in about 40 hours, or more than 1-5 mm. per hour. Drawn 

 by A. H. R. Buller and Ruth Macrae. Natural size. 



only after 42 hours but also after 50 hours, 52 hours, and 64 hours 

 respectively. At the end of 64 hours (early morning), clamp-con- 

 nexions were observed all around the part of the central mycehum 

 farthest from the {ab) inoculum, and diploidisation of the peripheral 



