244 



RESEARCHES ON FUNGI 



(aB) were obtained, and the haploid mycelia of each set were used 

 to make up the two possible kinds of diploid mycelia {AB)-i'{ab) 

 and {Ab)-\-{aB). Thus, altogether, there were in hand twelve 



Fig. 134. — Coprinus lagopus. Method of pairing mycelia of varioiis kinds with 

 a view to observing hyphal fusions between them. The Petri dish contained 

 cleared dung-agar. It was then inoculated with the following three pairs 

 of mycelia : {ah) and {Ab), lower pair ; {ah) and {a'h'), upper left-hand 

 pair ; {ah) and {aB), upper right-hand pair. The smaller mycelium of each 

 pair is {ah). Photograph taken three days after inoculation. The two 

 members of each pair, by means of hyphae in and in contact with the 

 dung-agar, had met and had begun to form hyphal fusions with one 

 another. Natural size. 



myceUa, six belonging to each of the two sets. The two sets of 

 myceha may be distinguished as follows : 



{AB), {ah), {Ah), {aB), {AB)+{ah), and {Ah)+{aB) 

 {A'B'), {a'h'), {A'h'), {a'B'), {A'B')+{a'h'), and {A'h')-^{a'B') 

 In order to obtain evidence whether or not any two of the 

 twelve myceha, when paired together, can form hjrphal fusions, 

 twenty- one of the sixty-six mathematically possible pairings were 

 made on dung-agar in Petri dishes (Fig. 134), and hyphal fusions 

 were sought for in the zone where the two members of each pair 



