246 RESEARCHES ON FUNGI 



of the nuclei in the haploid mycelium, it can be said that the 



Fig. 135. — Coprinus Ingopus. Camera-lucida drawing showing hyphal fusion3 

 between a diploid mycelium {Ab)^{aB) and a haploid mycelium (.46). The 

 two mycelia were set near to one another on a dung-agar plate ; two days 

 later they had grown into contact witli one another, and tlien the drawing 

 was made : a-a and 6-6, two radial hyphae of the diploid mycelium ; c-c, 

 d-d, e-e, f-f, g-g, and h-h, all radial hyphae of the haploid mycelium ; i-i and 

 j~j, two long lateral tangential branches of radial hyphae of the haploid 

 mycelium. The arrows indicate the direction of growth of the hyphae. 

 A line across a hypha, as at the letter g in the centre of the illustration, indicates 

 that a continuation of the hypha was seen but lias not been drawn. The 

 diploid hypha a-a : branch no. 1 is bent at the end as if about to fuse with 

 h-h ; branch no. 2 has fused with g-g ; and the end has fused with a branch 

 of d-d. The diploid hypha 6-6 : branch no. 1 has fused with a branch of 

 j-^ ; branch no. 2 with another branch of j-j ; branch no. 3 with i-i ; branch 

 no. 4 with a branch of /-/ ; and the end with a branch of dr-d. Thus the 

 diploid hyphae a-a and 6-6 have fused (anastomosed) in seven places with 

 the haploid hyphae c-c to j-j. The haploid hyphae have simple septa, no 

 clamp -connexions, and wide-angled branching, while two of them, h-h and 

 i-i, each bear an oidiophore. The oidiophore projects above the culture 

 medium and bears oidia in a drop of liquid, while the oidiophore p is immersed 

 in, and bears oidia in, a film of moisture on the surface of the culture medimn. 

 The haploid mycelium is becoming converted into a three-dimensional net- 

 work : a branch of d-d has fused with g-g, and a branch of that branch has 

 fused with j-j : j-j has also fused with g-g. The diploid hyphae bear clamp- 

 connexions but no oidiophores, and they have a narrow-angled mode of 

 branching. Drawn by A. H. R. Buller and Ruth Macrae. Magnification, 88. 



haploid mycelium becomes diploidised by a diploid mycelium with 

 which it is theoretically compatible. 



