256 



RESEARCHES ON FUNGI 



The twenty-four large haploid mycelia were inoculated as 

 follows : (1) one of each kind with a legitimate haploid mycehum ; 

 (2) one of each kind with a legitimate diploid mycehum ; (3) one 



of each kind with an ille- 

 gitimate haploid mycehum ; 

 (4) one of each kind with 

 another illegitimate haploid 

 mycelium ; (5) one of each 

 kind with an illegitimate 

 diploid mycehum ; and, 

 finally, (6) one of each kind 

 with two separated illegiti- 

 mate haploid myceha and 

 with an illegitimate diploid 

 mycehum made by combin- 

 ing two illegitimate haploid 

 myceha at the. periphery of 



Fig. 137. — Coprinus lagopus. Method of 

 making illegitimate combinations in one 

 set of experiments. The large haploid 

 mycelium (AB), growing on dung-agar 

 in a Petri dish, has attained a diameter the large haploid mycelium. 

 of 4-5 cm. At a little distance away ^, . i^: j ^f combin- 



fromitsperipheryhavejust been set four ±nus SIX Kmas Ol comuiii 



inocula, the size and position of which ations were made with each 



are indicated by the black spots. The i . i r i 



two lower inocula (^6) and (aB) quickly of the four kmds Ot large 

 met and diploidised one another, and , i -i ^YivopHa I A E) (ah) 

 this diploid mycelium, on coming into haplOld myceUa [JiJi), [aO), 

 contact with the large central (AB) /^^\ g^j^^j (aB). 

 mycelium, soon began to diploidise the ^ 

 (^J5) mycelixmi. The upper isolated (Ab) 

 and {aB) inocula, which were too far 

 distant from one another to meet and 

 fuse, on coming into contact with the 

 large central (AB) mycelitun failed to 

 diploidise the (AB) mycelium ; but, 



Ztr^^ceSi Tirm'^eitritS of considerable importance 



this had itself become diploidised. ^^^ ^j^^ solution of the prob- 

 Natural size. 



lem under investigation. 

 It may best be described by reference to one of the actual combina- 

 tions : the large haploid mycehum (AB) inoculated with the two- 

 illegitimate haploid myceha (^6) and {aB) and with the illegitimate 

 diploid mycelium {Ab)+{aB). When the large haploid mycelium 

 {AB) had attained a diameter of about 4-5 cm., it was inoculated 

 at its periphery, as shown in Fig. 137, at three widely separated 

 places : at one place was set down a fragment of an {Ab) mycehum, 



The sixth kind of com- 

 bination requires to be de- 

 scribed in greater detail, as 

 results obtained from it are 



