THE TRANSLOCATION OF PROTOPLASM 



99 



pores in the multiseptate macroconidia (fuseaux) of Microsporon 

 felineum, and he kindly demonstrated them to me (Fig. 55). 



My own observations x on the mode of formation of particular 

 septa in living myceliaof Pyronema confluens, Rhizoctonia solani, 

 and Rhizopus nigricans have convinced me of the correctness of 

 Wahrlich's conclusion that the septa of fungi in general are formed 

 by annular ingrowths from 

 a lateral wall. Further 

 evidence of this mode of for- 

 mation of septa is provided 

 by Dr. Gregory's compara- 

 tive observations on the 

 structure of the macroconidia 

 of Microsporon felineum in 

 various stages of develop- 

 ment. The spores were fixed 

 in glacial-acetic acid, washed, 

 and mounted in glycerine- 

 jelly containing methylene- 

 blue. The protoplasm of the 

 spores took on a blue colour 

 while the cell-walls remained 

 colourless. Dr. Gregory, on 

 examining spores treated in 

 this way, found several in 

 which the septa were only 

 partially grown and in which 

 it was evident that each of 



the septa had arisen in the first place as an annular ingrowth from 

 the exterior spore-wall (Fig. 55, C). 



From the investigations on protoplasmic continuity which have 

 just been summarised we may conclude that Ascomycetes, Basidio- 

 mycetes, and Fungi Imperfecti resemble Phanerogamia, Pteridophyta, 

 Muscineae, and multicellular Algae in that all the living cells which 

 make up an individual plant are connected together so as to form a 

 single mass of protoplasm. A realisation of this important fact 



1 Vide infra. 



Fig. 54. — Protoplasmic bridges extending 

 from cell to cell through the pores of 

 septa in mycelial hyphae. A, Fimetaria 

 fimicola, a Pyrenomycete ; B, Rhiz- 

 octonia solani. (= Corticium solani), a 

 Hymenomycete. Hyphae treated with 

 iodine and chlor-zinc iodine. Mag- 

 nification, 1030. 



