SPOROBOLOMYCES 



*75 



again obtained S. roseus, on this occasion from Water Lily leaves 

 gathered at Kenora on the Lake of the Woods. 



In 1925, Kluyver and van Niel, at my request, kindly sent me 

 cultures of Sjjorobolomyces roseus, S. salmonicolor , and S. tenuis so 

 that I have been able to study the production and liberation of the 



Fig. 85. — Sporobolomyces roseus. Spore-deposit made from the 

 yeast colony shown in Fig. 84 by inverting the Petri dish 

 for a few days. To prevent condensation of water-vapour, 

 the base of the dish was raised above the cover by means of 

 two glass slides placed on opposite sides of the cover. The 

 whole was then covered with a large crystallising dish. The 

 spore-deposit is S-shaped and is a mirror-picture of the 

 colony which produced it. Photographed by reflected sun- 

 light against a black background. Reduced to six-sevenths 

 the natural size. 



spores of these fungi at first hand. My observations on the drop- 

 excretion mechanism confirm those of Kluyver and van Niel. These 

 authors x stated that only one spore is produced on each sterigma 

 as in the Hymenomycetes ; but, by watching single yeast cells for 



1 A. J. Kluyver and ('. B. van Niel, loc. cit., p. 18. 



