SPOROBOLOMYCES 195 



sterigma then branched. The branch, supposed to have originated 



Fig. 98. — Sporobolomyces roseus. Spores, and nuclear phenomena during their 

 germination. Cultures made on malt-agar. Cells fixed with formalin-acetic- 

 alcohol and stained with iron-alum haematoxylin. A, spores which were shot 

 from their sterigmata on to malt-agar and were fixed prior to germination ; 

 each contains a single nucleus with a nucleolus ; a, two spores without a 

 vacuole ; b, two spores with a vacuole ; in a and b the base of each spore 

 looks downwards. B, spores germinating and producing yeast cells : a, a 

 spore with a young and, as yet, unnucleated bud ; b and c, two spores each with 

 an older bud, the nucleus has passed to the junction of the spore and the bud 

 and is there dividing ; d and e, two spores each of which has produced a bud 

 which has recently separated from it, the nucleus of the bud is smaller than 

 that of the spore ; /, a spore which has produced two buds in succession, each 

 of the three cells contains a single nucleus. Drawn by A. H. R. Buller and 

 Ruth Macrae. Magnification, 2234. 



in this way, is now producing a spore, and normal nuclear division 

 between the spore and the sterigma is in progress. Finally, the old 



