TILLETIA TRITICI 247 



malt-agar in the dish germinate rapidly and in large numbers, so 

 that numerous well-distributed new mycelia, derived entirely from 

 basidiospores, come into existence within a few days (Fig. 120). 

 The new culture may now be designated sub-culture No. 1. 



Oatmeal-agar or Leonian agar may be used instead of malt-agar 



Fig. 120. — Tilletia tritici. The basidiospore-discharge method of making 

 pure cultures. Basidiospores were allowed to fall from a mycelium in 

 an inverted agar dish on to the surface of the malt-agar in the Petri 

 dish here shown {cf. Fig. 119). The photograph shows the appearance 

 of the mycelium derived from the basidiospores eight days after 

 inoculation. Natural size. 



as the nutrient medium in making sub-culture No. 1. On any of 

 these media the young mycelia grow well and, in the course of two 

 to three weeks, form continuous white mats. 



The mycelium derived from the basidiospores in sub-culture 

 No. 1 progresses in its development so rapidly that, 24 hours after 

 inoculation, sub-culture No. 1 can be used to make a sub-culture 



