TILLETIA TRITICI 251 



and were then allowed to dry. When the spores fell upon such a 

 slide, they never germinated. 



The spore-deposit shown macroscopically in Fig. 122 — the first 

 Smut spore-deposit ever illustrated — was obtained as follows. A 



Fig. 124. — Tilletia tritici. A photomicrograph of a dry basidiospore-deposit 

 like that shown in Fig. 122. The spores fell in moist air and did not double 

 up in falling. They vary considerably in size. Magnification, 110. 



vigorously-growing test-tube culture (Fig. 121), made in the manner 

 already described, was inverted over a copper-sulphated glass slide 

 for three days, the combination being covered with a bell- jar to 

 prevent desiccation of the agar and of the mycelium growing upon 

 it. There were tens of thousands of hyaline sickle-shaped basidio- 

 spores in the deposit. A denser basidiospore-deposit is illustrated 



