140 



J. E. BRUGGER 



on the influence of inhibitors on the intensity of chemiiuminescence 

 and on the quenching influence of carbon dioxide." 



A presentation of other data obtained in this study, as well as a 

 theoretical consideration of all the phenomena of chemiiuminescence, 

 is in preparation. 



Discussion 



Rabinowitch: It should be reemphasized that you used pulsed light to irradiate 

 your algae. 



Arnold: We measured the chemiiuminescence of Krall's barley and found 

 spikes eight times higher than the steadj' state. This agrees with your observa- 

 tions for Chlorella in air containing 2% carbon dioxide. 



Witt: Did you observe the high spikes when you resumed pulsed irradiation 

 after having kept the Chlorella in the dark for a minute or so? 



Brugger: No. I obtained the highest spikes with Chlorella samples which had 

 been kept in the dark for an hour or so. If I stopped the irradiation after the 

 steady state had been reached and allowed the Chlorella to remain in the dark for 



Fig. 7. Recording monochromatic photometer: (front, left to right) Beckman 

 DU monochromator, cell and filter holder fitted with flow-type "Lucite" cell, 

 housing for photomultiplier tube and battery power supply. Not shown: photo- 

 signal amplifier and recorder, device for irradiating algae, apparatus for circulating 

 algal suspension through cell. 



