Phosphate in the Photosynthetic Cycle in 



Chlorella* 



E. C. WASSINK, Laboratory of Plant Physiological Research, Agricultural 

 University, Wageningen, Netherlands] 



Studies on light-dependent phosphorylation in photosj'nthesizing 

 organisms {Chromatium, strain D, and Chlorella) have been carried out 

 in this laboratory since 1947 (1-3). With Chromatium, so far, only an 

 introductory investigation was made; the more extensive part of the 



yq P/ml 



5 6 



Time m hours 



Fig. 1. Changes in the TCA-sohible phosphate of a suspension of Chlorella at 

 pH 4.0, in light and darkness, and in the presence and absence of COj. t Shift 

 to light, i shift to darkness. Expt. of May 30, 1950 (lb). 



work has been carried out with Chlorella. The general trend of the 

 phenomena is similar in both organisms (Fig. 1; cf. also refs. la 

 and lb). The folloA\'ing presentation deals especially with the Chlorella 

 work. 



It has been shown in experiments of long duration (several hours) 



* A brief report along this line has also been given at the .3rd International 

 Congress of Biochemistrj- at Brussels, 1955 (see RisunUs Communics., No. 11-lG, 

 p. 103). 



t 132nd Communication of this Laboratory; 44th Comm. on Photosynthesis. 



333 



