MOREIRA and VERNBERG: SYNERGISTIC EFFECTS OF ENVIRONMENTAL VARIABLES 



15 



20 25 



Temperature °C 



30 



15 



20 25 



Temperature °C 



15 



20 25 



Temperature "C 



Figure 2. — Response-surface estimation of percent metabolic 

 rate of Euterpina acutifrons maintained at 25 different tempera- 

 ture and salinity combinations. A. Copepods from Sao Sebastiao 

 maintained in Sao Sebastiao water. B. Copepods from Santos 

 maintained in Santos water. C. Copepods from Sao Sebastiao 

 maintained in Santos water. 



Table 3. — Analysis of variance of data for 

 Euterpina acutifrons (Sao Sebastiao animals 

 in Santos water, 28%o). T = temperature, S = 

 salinity. 



All of the analyzed factors, i.e., linear tempera- 

 ture and salinity, as well as the quadratic effect of 

 these factors, and the temperature-salinity in- 

 teraction, contributed significantly (0.057f level) 

 to the observed variability in respiration rates 

 (Table 3). A total of 62% of the variability could be 

 explained by these various factors. Figure 2C 

 shows the response surface contours fitted over the 

 experimental design. 



Animals From Sao Sebastiao in 

 Sao Sebastiao Diluted Water 



In this series the respiration experiments were 

 run at three temperatures to test whether or not 

 the results obtained for Santos animals were the 

 result of acclimation to a lower salinity. The respi- 

 ration rates at 28%o were essentially the same as 

 those for animals maintained in undiluted Sao 



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