HIROSE, K. - 



1976. Endocrine control of ovulation in medaka iOryzias 

 latipes) and ayu iPlecoglossus altivelis). J. Fish. Res. 

 Board Can. 33:989-994. 

 HiROSE, K., AND R. ISHin.A. 



1974. Effects of Cortisol and human chorionic gonadotrophin 

 (HCG) on ovulation in ayuPlecoglossus altivelis (Temminck 

 & Schlegel) with special respect to water and ion bal- 

 ance. J. Fish Biol. 6:557-564. 



Leitritz, E., and R. C. Lewis. 



1976. Trout and salmon culture (hatchery methods). Calif. 

 Dep. Fish Game, Fish Bull. 164, 197 p. 

 LUX, F. E. 



1969. Length-weight relationships of six New England 

 flatfishes. Trans. Am. Fish. Soc. 98:617-621. 

 Lux, F. E., AND F. E. NICHY. 



1969. Growth of yellowtail flounder, L/wo/!(/o ferruginea 

 (Storer), on three New England fishing grounds. Int. 

 Comm. Northwest Atl. Fish. Res. Bull. 6:5-25. 



Pitt, T. K. 



1971. Fecundity of the yellowtail flounder (Limanda fer- 

 ruginea) from the Grand Bank, Newfoundland. J. Fish. 

 Res. Board Can. 28:456-457. 

 ROYCE, W. F., R. J. BULLER, AND E. D. PREMETZ. 



1959. Decline of the yellowtail flounder {Limanda fer- 

 ruginea) off New England. U.S. Fish Wildl. Serv., Fish. 

 Bull. 59:169-267. 



Shehadeh, Z. H., and J. N. Ellis. 



1970. Induced spawning of the striped mullet Mugil 

 cephalus L. J. Fish Biol. 2:355-360. 



SINHA, V. R. P. 



1971. Induced spawning in carp with fractionated fish 

 pituitary extract. J. Fish Biol. 3:263-272. 



Smigielski, a. S. 



1975a. Hormone-induced spawnings of the summer floun- 

 der and rearing of the larvae in the laboratory. Prog.- 

 Fish Cult. 37:3-8. 



1975b. Hormonal-induced ovulation of the winter floun- 

 der, Pseudopleuronectes americanus. Fish. Bull., U.S. 

 73:431-438. 



Alphonse S. Smigielski 



Northeast Fisheries Center Narragansett Laboratory 

 National Marine Fisheries Servece, NOAA 

 R.R. 7A, Box 522 A 

 Narragansett. RI 02882 



TRACE METAL CONTAMINATION OF 



THE ROCK SCALLOP, HINNITES GIGANTEUS, 



NEAR A LARGE SOUTHERN 



CALIFORNIA MUNICIPAL OUTFALL' 



Los Angeles County's submarine discharge of 

 municipal wastewater from the Joint Water Pol- 

 lution Control Plant (JWPCP) off Palos Verdes 

 Peninsula is the single largest anthropogenic 



source of trace metals to the marine ecosystem off 

 southern California. The 1974 annual mass emis- 

 sion rates of chromium, copper, and zinc via this 

 discharge (4.8 x 10" 1/yr, which underwent pri- 

 mary treatment only) were about 400, 300, and 

 850 t, respectively; these were approximately 10 

 times the corresponding inputs measured in 

 1971-72 surface runoff from southern California 

 ( Young et al. 1973). As a result, bottom sediments 

 around this submarine outfall system are highly 

 contaminated by a number of trace metals (Gallo- 

 way 1972; Young et al. 1975). Here we report ab- 

 normal levels of seven metals in three tissues of 

 the filter-feeding rock scallop, Hinnites gigan- 

 teus,^ that was collected in the discharge zone and 

 thus had been exposed to suspended wastewater 

 particulates. (The adductor muscle of this bivalve 

 mollusc is considered to be a delicacy, and scallops 

 near the discharge are sought by sport divers.) 



Procedures 



During 1974, divers collected eight scallops 

 within the size range generally consumed ( 10 to 25 

 cm in diameter) from depths of about 20 m at three 

 stations in the discharge zone between Whites 

 Point and Point Vicente: these stations were <1 

 km off Palos Verdes Peninsula. Six scallops in the 

 same size range also were taken from control sta- 

 tions at similar depths off Santa Catalina and 

 Santa Barbara Islands (Figure 1). To check our 

 1974 results, during 1976 eight specimens within 

 this size range were again collected from this re- 

 gion in the discharge zone. However, we were not 

 able to obtain additional island samples; there- 

 fore, five specimens were collected from each of 

 two coastal stations located approximately 50 km 

 to the north and south of Palos Verdes Peninsula. 

 The samples were frozen in plastic bags after col- 

 lection. Later, digestive gland, gonad, and adduc- 

 tor muscle tissues were excised from each speci- 

 men before it was fully thawed, using a new 

 carbon steel scalpel and a cleaned Teflon^ sheet; 

 the tissues were placed in cleaned polyethylene 

 vials. Care was taken to avoid contaminating the 

 gonadal or muscle tissue samples with sediments 

 or juices from the digestive glands. 



Following dissection, each sample ( 1 to 2 g wet 

 weight) was digested in 10 ml of a 1:1 nitric acid 



'Contribution No. 85 of the Southern California Coastal Water 

 Research Project. 



'^Formerly Hinnites multirugosus (Roth and Coan 1978). 

 ■''Reference to trade names does not imply endorsement by the 

 National Marine Fisheries Service, NOAA. 



936 



FISHERY BULLETIN: VOL. 76. NO. 4. 1979. 



