Nkw York Agkicultdkal Expkkiment Station. 185 



meter; the back left quarter had slightly less; the front loft 

 quarter had often less than 100 per cubic centimeter, and the 

 front right quarter but little more. 



Making allowance for the work done by galactase, the milk 

 from different quarters of the udder of the above mentioned cow 

 should show different rates of chemical change proportional ta 

 the number of germs present in the respective quarters of the 

 udder, if these changes are to be associated with contamination 

 within the udder. 



The results already given in Table IV, under chloroform, 

 relate to this point. The quarters of the udder are there 

 designated as follows: II, front right; IV, front left; VI, back 

 right; VIII, back left. The second determination was made in 

 the presence of 4 per ct. of chloroform. In order to obtain 

 sufficient material for a large number of analyses, three sue* 

 cessive messes of milk were collected and united. Care was 

 taken to reject the fore-milk and keep out bacteria from other 

 sources. The following table shows the results in this test up 

 to 15 weeks: 



Table V b. — Soluble Nitrogen Formed in Milk from Different 



Quarters of Udder. 



milk drawn june 11 and 12. 



Soluble nitrogen in 100 lbs. total nitrogen. 



The results given in Tables IV and Vb show in a general 

 way that there is a relation between the numbers of bacteria 

 present in the udder and the rapidity with which the milk pro- 

 duced there undergoes self-digestion in the presence of chloro* 

 form or formalin. 



It may be held that the presence of these bacteria has merely 

 stimulated the production of an extra amount of galactase, but 



