164 



THE MICHIGAN ACADEMY OF SCIENCE. 



(2-.) 



It will be seen from Table 1 that the i^owdered germ substance of 

 Bacillus pyocpaneus is surely fatal to guinea-pigs when injected 

 intraperitoneally in the proportion of one part to 50,000 parts of hodj 

 weight. 



When injected subcutaneously in guinea-pigs, even in as large quan- 

 tity as one part of the toxin to 10,000 of body weight, the only incon- 

 venience that resulted was a slight swelling at the point of inoculation, 

 followed by temporary loss of weight. Subcutaneous injections did not 

 give immunity to subsequent intraperitoneal treatments. 



These results show that the toxin is not destroyed, although it is 

 slightly diminished at the temperatures given in the tables. 



TABLE 2. — Toxicity of the Germ Substance of Bacillus Pyocyaneus injected intraperi- 

 toneally into Guinea-pigs after being heated. 



(30 minutes at 100 C.) (5 + in 12 hours to 18 days, 1—.) 



