166 THE MICHIGAN ACADEMY OF SCIENCE. 



which may be extracted from the cell, either in the fresh state or after 

 treatment with alcohol and ether by physiologic salt solution. 



5. The mininnim lethal dose of the celhilar toxin is for guinea pigs, 

 when given intraperitoneally, about one part of the cell substance to 

 50,000 parts of body weight. 



6. When injected subcutaneously in guinea-pigs in the proportion of 

 one part of the cell substance to 10,000 j)arts of body weiglit or less, no 

 permanent ill-effect results. 



7. Subcutaneous injections do not give immunity to subsequent in- 

 traperitoneal injections. 



Donald R. MacIntyre. 



Assistant in Hygienic Laboratory, University of Michigan, 

 Ann Arbor. 



