New York Agricultural Experiment Station. Ill 



centimeter of milk was taken and spread over an area of 24 x 20 

 millimeters and compared to Skar's smear from which the fat was 

 removed, indicates that this contention is not true. 



To further determine whether the bacteria were lost in the washing 

 process, a drop of milk stained by Skar's method was dried in one 

 concavity of a clean, double, hollow-ground slide and treated for 

 some time with xylol. Then the xylol was carefully allowed to run 

 over into the second concavity and evaporated. Examination 

 under the microscope failed to reveal any bacteria. This operation 

 was repeated with ether, but no bacteria could be found. This, 

 together with the counts made, shows that if any bacteria are removed 

 in the process of preparing the slides the number is so few as to be of 

 little or no importance. 



Rosam's method. — A description of the technique of this method 

 is given on page 82. Table XV gives the results of a few com- 

 parative counts. 



Table XV. — Comparative 



Counts Obtained by 

 Methods. 



Rosam method. 



137,480,000 

 none 



60,000,000 

 149,000,000 

 165,000,000 



THE ROSAM AND BREED 



Breed method. 



601,400,000 

 863,000 

 172,000,000 

 412,000,000 

 564,000,000 



