138 Report of the Department of Bacteriology of the 



Table V. — Bacteria Present in the Udders of 43 op 



■ 



o 



802 

 803 

 804 

 806 



807 



808 



1 



CO 



P 



200 ± 

 200 ± 

 ? 



200 ± 



200 ± 

 32 



a 

 3 

 o 



CO 



E 



27 

 

 

 3 



17 

 23 



Number of Bacteria per Cubic Centimeter of Strippings. 



RIGHT FRONT QUARTER. 



el 

 3 

 o 



d 

 o 

 o 



CD 

 CO 



58 







430 



5 



27 

 45 



Notes, t 



All— M. 211.2233533 



RIGHT HIND QUARTER. 



a 



3 



o 



eg 



CO 



s- 





 6 

 

 1 



3 



12 



a 



3 



o 

 o 



-0 



a 

 o 



u 



CD 



CO 



2 

 6 

 1 

 8 



5 

 46 



Notes, f 



* The agar plates were first incubated for five days at 20-23 degrees C. and colo- 

 nies counted; then again incubated for two days at 37 degrees C, for second count. 



t The first number given under " Notes " shows how many colonies on the agar 

 plates had the same general appearance as the colony from which a culture was isolated. 

 The group number following was determined in each case from this single isolated 

 colony. 



At the same milking that furnished the samples for study of the 

 cell content, samples of strippings were collected in sterile test tubes, 

 from each quarter of the udder of forty-three of these cows by Mr. 

 Wilson and Mr. Ruehle. With the help of Dr. Harding, these 

 samples were plated in duplicate in lactose agar using one-half cubic 

 centimeter of undiluted milk for each plate. Mr. Wilson studied the 

 udder flora as follows: The plates were incubated for five days at 

 20-23 degrees C. and counted; then again incubated at 37 degrees C. 

 for two days and recounted. Sixty-one cultures were isolated and 

 studied as described in Technical Bulletin No. 27. The results of the 

 tests made to determine the group numbers of these cultures are given 

 in Tables IX and XII of the bulletin referred to. The details of 

 the counts obtained and the cultures isolated from each quarter 

 are given in Table V of the present bulletin. 



Since the meaning of the group number as here used is not familiar 

 to everyone, the tabular statement opposite, taken from the standard 

 card recommended by the Society of American Bacteriologists, is 

 given : 



