New York Agricultural Experiment Station. 141 



micrococci found generally on the skin of man and animals. Many 

 bacteriologists believe these to be indistinguishable from the pus- 

 forming cocci generally spoken of as Micrococcus (Staphylococcus) 

 pyogenes. 



The two strains of streptococci isolated from udders as given by 

 Harding and Wilson in Technical Bulletin No. 27 were both isolated 

 from this herd at this time. One of these with the group number 

 Str. 222.2223033 appeared as an apparently pure culture in the 

 right hind quarter of Cow 242. This quarter gave a bacterial 

 count of 16,610 per cubic centimeter which was the highest obtained 

 from any quarter of any cow in the herd. The left hind quarter 

 of the same cow gave a bacterial count of 5280 per cubic centimeter 

 but this was due to an apparently pure culture of a yellow chromo- 

 genic micrococcus which, when isolated, gave the group number 

 M. 211.2223533. The interesting fact to note was that the cell 

 count of this cow was high, namely 3,440,000 per cubic centimeter. 



The second streptococcus, which had the group number Str. 

 211.2223033, was apparently present in pure culture in both the 

 right hind and the left front quarters of Cow 329. The bacterial 

 counts for these two quarters were 3600 and 1340 per cubic centi- 

 meter respectively. The cell count for this cow was likewise high, 

 namely, 3,810,000 per cubic centimeter. 



The characteristics of the colonies which grew on the plates 

 inoculated with milk from the right hind quarter of Cow 7 (bacterial 

 count of 1123) were such that there is good reason for thinking 

 that the form present in this case was a streptococcus. The culture 

 isolated was lost because of failure to grow well on ordinary media, 

 a further characteristic of the streptococci. The cell count for this 

 cow was 6,950,000 per cubic centimeter which was the highest 

 found for any cow in this herd. 



The fact that the three cows known or suspected to have had a 

 streptococcic infection of the udder gave three of the highest cell 

 counts in the herd is suggestive especially when combined with 

 what other investigators have reported. Nevertheless the fact 

 that there were two other cows (Nos. 616, 803) which gave equally 

 high counts while there is little or no reason for suspecting that a 

 streptococcic infection existed compels caution in the interpretation 

 of this result. More data of this sort must be secured before the 

 real condition of affairs will become evident. 



